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Real Time PCR Kit for Monkeypox Virus (PCR-Fluorescence Probing)
Real Time PCR Kit for Monkeypox Virus is based on real-time PCR technology. This kit adopts real time fluorescence PCR technology, which is suitable for nucleic acid detection of monkeypox virus extracted from clinical samples such as Human serum, lesion exudate samples and swab specimens Each reaction system contains specific primers and fluorescent probes to detect the virus, and the monkeypox virus nucleic acid can be qualitatively detected by collecting the fluorescent signal generated by PCR amplification.
Monkeypox is a rare zoonotic disease characterized by skin eruptions caused by the Monkeypox virus (MPV) and whose clinical symptoms are similar to smallpox. It can be transmitted through contact with bodily fluids, lesions on the skin or on internal mucosal surfaces, such as in the mouth or throat, respiratory droplets and contaminated objects. Detection of viral DNA by polymerase chain reaction (PCR) is the preferred test for monkeypox.
Specification
Product Name | Monkeypox Virus Real Time PCR Kit |
Detection Channels | FAM |
IC | VIC |
Ct Value | 35 |
Limit of Detection | 200 Copies/mL |
Storage | -20℃±5℃ |
Transportation | Under frozen condition |
Shelf Life | 12 Months |
Sample Type | Nasal swabs, oropharyngeal swab, saliva, urine, lesion tissue, exudate, and blood, etc. |
Package | 24 Tests/Kit,48 Tests/Kit,96 Tests/Kit |
Components | PCR Reaction Buffer, PCR Enzyme Mixture, Positive control, Negative control |
Real-Time PCR System: Molarray MA-6000, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM 5,Hongshi SLAN-96S/96P, AGS8830, AGS4800.
COMPONENTS
Specifications | LQ-000301 24T | LQ-000302 48T | LQ-000303 96T |
PCR Reaction Buffer | 336μL×1 tube | 672μL×1 tube | 672μL×2 tube |
PCR Enzyme Mixture | 30μL×1 tube | 50μL×1 tube | 100μL×1 tube |
Positive control | 50μL×1 tube | 100μL×1 tube | 200μL×1 tube |
Negative control | 50μL×1 tube | 100μL×1 tube | 200μL×1 tube |
Instruction for use (pcs) | 1 | 1 | 1 |
PROCEDURES
Sample Collect- Nucleic Acid Extraction-Reaction Mix Preparation-Run the Real-Time PCR Program-Get the Result
PRODUCT PERFORMANCE INDEX
1. Sensitivity: 200 copies/mL.
2. Specificity: No cross reaction with Enterovirus (EV), Measles virus (MV), Rubella virus (RV), Varicella-zoster virus (VZV), Dengue virus (DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), human herpes virus 6(HHV-6), etc.
3. Precision: CV ≤ 5%.